Inhibitory Effects of a Novel Nutrient Mixture on MMP Secretion and Invasion on Human Thyroid Cancer Cell Line SW 579

Thyroid cancer is the most common endocrine malignancy. Mortality from thyroid cancer results from tumor invasion with local and distant metastases. Degradation of extracellular matrix is the hallmark of metastasis and is mediated by matrix metalloproteinase (MMP) enzymes. A novel Nutrient Mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract has exhibited significant anticancer activity in other cancer cell lines. In this study, we investigated the effects of NM on a thyroid cancer cell line on proliferation (MTT Assay), MMP secretion (Gelatinase-Zymography), MatrigelTM invasion, and morphology (H&E). Zymography demonstrated that NM inhibited MMP-2 secretion, with virtually total inhibition at 1000 mg/mL. MatrigelTM invasion was inhibited at 50, 100, and 500 mg/mL by 42%, 63%, and 100%, respectively. NM was nontoxic to thyroid cancer cells below 500 mg/mL, and H&E staining did not show morphological changes.